Karakteristik Fraksi Aktif Biopigmen Fukosantin Rumput Laut Cokelat sebagai Antioksidan dan UV-protector

Characteristics of Fucoxanthin’s Active Fraction from Brown Seaweeds Biopigments as Antioxidants and UV Protectors

  • Asadatun Abdullah Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, IPB University
  • Nurjanah Nurjanah Departemen Teknologi Hasil Perairan, FPIK IPB University
  • Ade Irma Suryani Nasution Departemen Teknologi Hasil Perairan, FPIK IPB University
Keywords: Padina sp., Sargassum sp., SPF, total phenol

Abstract

Padina sp. and Sargassum sp. contains pigment namely fucoxanthins and have antioxidants, anticarcinogenic, and anti-UV. The objectives of this study were to determine the optimal concentration of acetone to produce the best fucoxanthin active fraction with antioxidant, total phenol, SPF value functions as well as to identify the profile of the compound with LC-MS/MS. Seaweeds were immersed in 10% DMSO before extracted with 80% and 90% acetone solvent. The results of antioxidant DPPH, FRAP, and ABTS were Padina sp. of 153.55 ppm, 94.22 μmol Fe2+/g, 66.43 ppm (80% acetone) and 65.76 ppm, 93.11 μmol Fe2+/g, 78.33 ppm (90% acetone); Sargassum sp. of 131.37 ppm, 103.67 μmol Fe2+/g, 57.29 ppm (80% acetone) and 103.24 ppm, 71.72 μmol Fe2+/g, 61.45 ppm (90% acetone). Total phenol of 80% dan 90% acetone from Padina sp. were 675.48 mg GAE/g and 852.90 mg GAE/g; Sargassum sp. of 565.81 mg GAE/g and 135.16 mg GAE/g. SPF value with optimal concentration 50 mg/mL had resulted of Padina sp. by 9.08 (80% acetone) and 17.10 (acetone 90%); Sargassum sp. of 7.56 (acetone 80%) and 3.56 (acetone 90%). Acetone 80% is the optimal solvent to produce the best fucoxanthin active fraction with the highest characteristics of yield, antioxidant activity, total phenol content, and SPF value.

References

Apostolidis E, Lee CM. 2010. In vitro potential of Ascophyllum nodusum phenolic antioxidant-mediated alpha-glycosidase and alpha-amylase inhibition. Journal of Food Science. 75(3) : 97-102.

Apak R, Guclu K, Ozyurek M, Celik SE. 2008. Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay. Microchimica Acta.(160) : 413-419.

Badarinath AV, Rao KM, Chetty CMS, Ramkanth S, Rajan TVS, Gnanaprakash K. 2010. A review on in-vitro antioxidant methods : comparisons, correlations and considerations. International Journal of Pharmatech Research. 2(2) : 1276-1285.

Boeing JS, Erica OB, Beatriz CS, Paula FM, Vitor CA, Jesui VV. 2014. Evalution of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the barriers : Application of principal component analysis. Chemistry Central Journal. 8(48) : 1-9.

[BPOM] Badan Pengawas Obat dan Makanan. 2004. Monograft Ekstrak Tumbuhan Obat Indonesia. Jakarta (ID) : BPOM RI.

Dang TT, Bowyer MC, Altena IAV, Scarlett CJ. 2018. Comparison of chemical profile and antioxidant properties of the brown algae. International Journal of Food Science and Technology. 53 : 174-181.

Diachanty S, Nurjanah, Abdullah A. 2017. Aktivitas antioksidan berbagai jenis rumput laut cokelat dari perairan Kepulauan Seribu. Jurnal Pengolahan Hasil Perikanan Indonesia. 20(2) : 305-318.

Denantica DP. 2012. Analisis faktor-faktor yang mempengaruhi ekspor rumput laut dan kajian trend volume ekspor rumput laut Indonesia ke China. [skripsi]. Bogor (ID) : Institut Pertanian Bogor.

Departement of Health and Human Services. 2000. Report on Carcinogens Background Document for Broad-Spectrum Ultraviolet (UV) Radiation and UVA, UVB, and UVC. North Carolina (USA) : Departement of Health and Human Services.

Egwaikhide PA, Gimba CE. 2007. Analysis of the phytochemical content and antimicrobial activity of Plectranthus glandulosis whole plant. Middle-East Journal of Scientific Reasearch. 2(3-4) : 135-138.

Fernandes T. 2018. Karakterisasi dan identifikasi senyawa aktif ekstrak pigmen Sargassum polycystum dan Padina minor. [skripsi]. Bogor (ID) : Institut Pertanian Bogor.

Fung AYC. 2012. The fucoxanthin content and antioxidant properties of Undaria pinnatifida from Marlborough Sound, New Zealand. [thesis]. Auckland University of Technology University.

Geraldino PJL, Liao LM, Boo SM. 2005. Morphologicl study of the marine algal genus Padina (Dictyotales, Phaeophyceae) from Southern Philippines : 3 species new to Philippines. Algae. 20(2) : 99-112.

Javanmardi J, Stushnoff C, Lockeb E, Vivancob JM. 2003. Antioxidant activity and total phenolic content of iranian ocimum accessions. Food Chemistry. 83 : 547-550.

Johnsen G, Lyssa P, Aamodt K. 2014. Sunscreen compositions comprising carotenoids. Foreign Application Priority Data. Patent Application Serial no. 051365.1. 1-21.

Khaled N, Hiba M, Asma C. 2012. Antioxidant and antifungal activities of Padina pavonica and Sargassum vulgare from the Lebanese Mediterranean Coast. Advances in Environmental Biology. 6(1) : 42-48.

[KKP] Kementerian Kelautan dan Perikanan. 2014. Komoditas Rumput Laut Kian Strategis. [6 April 2014]. Diperoleh dari http://www.news.kkp.go.id- diakses pada 30 Juli 2018.

Kumar S, Sandhir R, Ojha S. 2014. Evaluation of antioxidant activity and total phenol in different varieties of Lantana camara leaves. BMC Research Notes. 7(560) : 1-9.

Lestari IL, Mita SR. 2017. Review : Potensi alga laut dan kandungan senyawa biologisnya sebagai bahan baku kosmeseutikal. Farmaka. 4(3) : 1-15.

Limantara L, Heriyanto. 2010. Studi komposisi pigmen dan kandungan fukosantin rumput laut cokelat dari Perairan Madura dengan kromatografi cair kinerja tinggi. Ilmu Kelautan. 15(1) : 23-32.

Limantara L, Heriyanto. 2011. Optimasi proses ekstraksi fukosantin rumput laut coklat Padina australis Hauck menggunakan pelarut organic polar. Ilmu Kelautan. 16(2) : 86-94.

Narayani SS, Saravana S, Bharathiaraja S, Mahendran S. 2016. Extraction, partially purification and study on antioxidant property of fucoxanthin from Sargassum cinereum J. Agardh. Journal of Chemical and Pharmaceutical Research. 8(3) : 610-616.

Noviendri D, Jaswir I, Salleh MH, Taher M, Miyashita K, Ramli N. 2011a. Fucoxanthin extraction and fatty acid analysis of Sargassum binderi and S. duplicatum. Journal of Medicinal Plants Research. 5(11) : 2405–2412.

Nursid M, Noviendri D, Rahayu L, Novelita V. 2016. Isolasi fukosantin dari rumput laut coklat Padina australis dan sitotoksisitasnya terhadap sel MCF7 dan sel vero. JPB Kelautan dan Perikanan. 11(1) : 83-90.

Pan RN, Kuo BPC, Pao LH. 2012. Validated LC-MS-MS method for the determination of quetiapine in human plasma : application to a pharmacokinetic study. Journal of Chromatographic Science. 50 : 277-282.

Petro AJ. 1981. Correlation of spectrophotometric data with sunscreen protection factor. International Journal of Cosmetic Sciences. 185-196.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation-decolorizing assay. Free Radical Biol Med. 26 : 1231-1237.

Renhoran M, Noviendri D, Setyaningsih I, Uju. 2017. Ekstraksi dan purifikasi fukosantin dari Sargassum sp. sebagai anti-acne. Jurnal Pengolahan Hasil Perikanan Indonsesia. 20(2) : 370-379.

Sami FJ, Nur S, Martani MM. 2015. Uji aktivitas tabir surya pada beberapa spesies dari family Zingiberaeae dengan metode spektrofotometri. As-Syifaa. 7(2) : 164-173.

Seely GR, Duncan MJ, dan Vidaver WE. 1972. Preparative and analytical extraction of brown algae with dimethyl sulfoxide. Marine Biology. 12 : 184-188.

Sudhakar MP, Ananthalakshmi JS, Nair BB. 2013. Extraction, purification, and study on antioxidant properties of fucoxanthin from brown seaweeds. Journal of Chemical and Pharmaceutical Research. 5(7) : 169-175.

Suryana WN. 2010. Optimasi ekstraksi sinensetin dari daun kumis kucing. [skripsi]. Bogor (ID) : Institut Pertanian bogor

Tang C. 1991. Phenolic Compound in Food. Washington (USA) : American Chemical Society.

Wahyuni DT, Widjanarko SB. 2015. Pengaruh jenis pelarut dan lama ekstraksi terhadap ekstrak karotenoid labu kuning dengan metode gelombang ultrasonic. Jurnal Pangan dan Agroindustri. 3(2) : 390-401.

Widyastuti N. 2010. Pengukuran aktivitas antioksidan dengan metode CUPRAC, DPPH, dan FRAP serta korelasinya dengan fenol dan flavonoid pada enam tanaman. [skripsi]. Bogor (ID) : Institut Pertanian Bogor.

Wu HY, Lim SJ, Mustafha WAW, Maskat MY, Said M. 2014. Characterisation and stability of pigment extracted from Sargassum binderi obtained from Semporna, Sabah. Sains Malaysiana. 43(9) : 1345-1354.

Yan X, Chuda Y, Suzuki M, Nagata T. 1999. Fucoxanthin as the major antioxidant in Hijika fusiformis a common edible seaweed. Bi

Published
2021-06-11