Penghambatan fraksi fukoidan rumput laut cokelat (Sargassum polycystum dan Turbinaria conoides) terhadap α-amilase dan α-glukosidase

The Inhibition of Fucoidan Fraction from Sargassum polycystum and Turbinaria conoides to α-Amylase and α-Glucosidase

  • Widya Puspantari Pusat Teknologi Agroindustri, Badan Pengkajian dan Penerapan Teknologi, Jalan M.H. Thamrin Kota Jakarta Pusat, Jakarta
  • Feri Kusnandar Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor
  • Hanifah Nuryani Lioe Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor
  • Noer Laily Pusat Teknologi Agroindustri, Badan Pengkajian dan Penerapan Teknologi, Jalan M.H. Thamrin Kota Jakarta Pusat, Jakarta
Keywords: antidiabetes, berat molekul, fukoidan, ultrafiltrasi

Abstract

Fukoidan merupakan polisakarida tersulfat dalam rumput laut cokelat yang memiliki aktivitas biologis, di antaranya sebagai antidiabetes. Penelitian ini bertujuan untuk menentukan kemampuan fukoidan dari rumput laut cokelat (Sargassum polycystum dan Turbinaria conoides) sebagai antidiabetes, yaitu dalam menghambat aktivitas α-amilase dan α-glukosidase secara in vitro. Kemampuan penghambatan enzim dievaluasi dari ekstrak kasar fukoidan, hasil fraksinasi dengan membran ultrafiltrasi (10 dan 30 kDa), dan kromatografi filtrasi gel. Ekstrak kasar fukoidan (1 mg/mL) dari S. polycystum hasil ekstraksi asam, dan dari T. conoides hasil ekstraksi air menunjukkan aktivitas penghambatan terhadap α-amilase dan α-glukosidase yang tertinggi. Fraksi dengan berat molekul >30 kDa hasil ultrafiltrasi ekstrak fukoidan dari S. polycystum dan T. conoides (pada konsentrasi 50 mg ekstrak/3,0 mL akuabides) menunjukkan aktivitas penghambatan yang rendah terhadap α-amilase (16,88% dan 9,67%), tetapi tinggi terhadap α-glukosidase (99,06% dan 65,97%). Fraksinasi dengan kromatografi filtrasi gel menunjukkan adanya senyawa aktif dalam fraksi fukoidan yang memiliki aktivitas penghambatan yang tinggi terhadap α-glukosidase, yaitu fraksi dengan berat molekul 30-70 kDa untuk S. polycystum dan >70 kDa untuk T. conoides. Penelitian ini menunjukkan bahwa fraksi fukoidan dari S. polycystum dan T. conoides memiliki kemampuan penghambatan terutama terhadap α-amilase dan α-glukosidase, dan berpotensi sebagai antidiabetes.

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Published
2020-04-30