Profil Protein Daging Ikan Kurisi Merah (Pagrus major) yang Diberi Pakan dengan Tambahan Tepung Daun Zaitun (Olea europaea L.)

Profile Protein of Fish Red Sea Bream (Pagrus major) Muscle Fed with Additional Olive (Olea europaea L.) Leaf Powder

  • Muh Ali Arsyad Program Studi Teknologi Pengolahan Hasil Perikanan, Politeknik Pertanian Negeri Pangkajene dan Kepulauan
  • Arham Rusli Program Studi Teknologi Pengolahan Hasil Perikanan, Politeknik Pertanian Negeri Pangkajene dan Kepulauan
Keywords: miofibril, kolagen, polifenol, sarkoplasma, struktur daging

Abstract

Pemberian pakan dengan tambahan antioksidan dari bahan alam terbukti mampu meningkatkan kualitas daging yang dihasilkan. Aktivitas antioksidan dari bahan alam ditentukan oleh kandungan polifenol. Daun zaitun mengandung 6-9% polifeneol. Penelitian ini bertujuan untuk menentukan pengaruh penambahan tepung daun zaitun pada pakan terhadap profil protein daging ikan yang dikultur selama 25 hari pada akuarium dengan kondisi yang terkontrol. Komposisi protein daging dianalisis dengan SDS-PAGE dan kandungan protein sarkoplasma, miofibril dan kolagen dianalisa dengan metode biuret. Kandungan protein sarkoplasma dan miofibril tidak berbeda pada kedua kelompok ikan, tetapi kandungan kolagen daging ikan yang diberi pakan dengan tepung daun zaitun 1,63 kali lebih tinggi dibanding ikan dengan pakan kontrol. Mikrostruktur daging ikan dengan pakan tepung daun zaitun lebih kompak dibanding ikan dengan pakan kontrol, hal tersebut disebabkan kandungan kolagen yang lebih tinggi. Hasil penelitian ini menunjukkan bahwa penambahan tepung daun zaitun pada pakan mampu meningkatkan tekstur pada daging melalui peningkatan kuantitas dan kualitas serat kolagen pada endomisium.

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Published
2020-04-30