Pengaruh Kombinasi Perlakuan Air Panas dan Kultur Jaringan terhadap Infeksi Virus pada Bawang Merah

  • Astri Windia Wulandari Wulandari Balai Penelitian Tanaman Sayuran, Bandung 40391
  • Sobir Sobir Department of Agronomy, IPB University
  • Sri Hendrastuti Hidayat Department of Plant Protection, IPB University
Keywords: 'Bima Curut', Carlavirus, Potyvirus, 'Sumenep', virus free

Abstract

Effect of Combination of Hot Water Treatment and Tissue Culture on Virus Infections in Shallot

Most shallot farmers in Indonesia use bulbs as their propagative materials despite many diseases were known to be transmitted through bulbs. Heat treatment is a common method used to produce virus-free plants, i.e. using hot water or hot air treatment which is generally applied on the dormant organs such as seeds or bulbs. Research was conducted to get virus free shallot bulbs through hot water treatment combined with tissue culture. Detection of the virus before treatment using Dot immuno binding assay confirmed the infection of Potyvirus (Onion yellow dwarf virus/OYDV), Carlavirus (Shallot latent virus/SLV and Garlic common latent virus/GCLV) from bulb. The experiment was done using a completely randomized design with three factors, i.e. cultivar (Sumenep and Bima Curut), temperature (45 °C, 50 °C, and room temperature), and heating time (15 and 30 min).  In general, hot water treatment combined with tissue culture did not affect the ability of plantlets to grow and the number of leaf. Based on virus detection using RT-PCR, 100% elimination of Potyvirus and Carlavirus on cultivar Bima Curut was observed at temperature of 45 °C for 15 minutes; whereas on cultivar Sumenep hot water treatment at 50 °C for 15 minutes was able to eliminate 100% Potyvirus and 33.33% Carlavirus.

Downloads

Download data is not yet available.

References

Almaarri K, Massa R, Albiski F. 2012. Evaluation of some therapies and meristem culture to eliminate Potato Y Potyvirus from infected potato plants. Plant Biotechnology. 29:237–243. DOI: https://doi.org/10.5511/plantbiotechnology.12.0215a.

Anggraini S, Hidayat SH, 2014. Sensitivitas metode serologi dan polymerase chain reaction untuk mendeteksi Bean common mosaic potyvirus pada kacang panjang. J Fitopatol Indones. 10(1):17–22. DOI: https://doi.org/10.14692/jfi.10.1.17.

Budiarto K, Y. Sulyo, IB. Rahardjo, D. Pramanik. 2008. Pengaruh durasi pemanasan terhadap keberadaan Chrysanthemum Virus-B pada tiga varietas krisan terinfeksi. J Hort. 18(2):185–192.

Budiono DP. 2004. Multiplikasi in vitro tunas bawang merah (Allium ascalonicum L.) pada berbagai taraf konsentrasi air kelapa. J Agro. 8(2):75–80.

Doyle JJ, Doyle JJ. 1990. Isolation of plant DNA from fresh tissue. Focus. 12:13–15. DOI: https://doi.org/10.2307/2419362.

Duriat AS. 1990. Inventarization of pest and diseases on lowland vegetable in Madura, Bali dan Lombok. Bul Penel Hort. edisi khusus. 18:119–130.

Duriat AS, Sukarna E. 1990. Deteksi penyakit virus pada klon bawang merah. Bul Penel Hort. edisi khusus. 18:146–150.

Fajaro Thor VM, Nishijima M, Buso JA, Torres AC, Avila AC, Resende RO. 2001. Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil [Internet]. Fito Brazil 26(3); [diunduh 2011 Feb 6]. Tersedia pada: http:// www. Scielo.briscielo.php?pid=S0100-41582001000300007 script=sci arttext. DOI: https://doi.org/10.1590/S0100-41582001000300007.

Fletcher PJ, Fletcher JD, Lewthwaite SL. 2010. In vitro elimination of onion yellow dwarf and shallot latent viruses in shallots (Allium cepa var. ascalonicum L.). N Z J Crop Hortic Sci. 26(1):23–26. DOI: https://doi.org/10.1080/01140671.1998.9514035.

Gunawan LW. 1992. Teknik Kultur Jaringan Tumbuhan. Pusat Antar Univ. Bioteknologi. Bogor (ID): Institut Pertanian Bogor.

Gunaeni N, Wulandari AW, Duriat AS, Muharam A. 2011. Insiden penyakit virus tular umbi pada tigabelas varietas bawang merah asal Jawa Barat dan Jawa Tengah. J Hort. 21(2):164–172. DOI: https://doi.org/10.21082/jhort.v21n2.2011.p164-172.

Hadidi A, Khetarpal RK, Koganezawa H. 1998. Plant Virus Diseases Control. St. Paul (US): APS Pr.

Harti H, Sobir, Wiyono S, Hidayat SH. 2018. Perlakuan air panas pada umbi bawang merah (Allium cepa var. ascalonicum) untuk menekan infeksi virus di lapangan. J Hortikultur Indones. 9(3):149–157. DOI: https://doi.org/10.29244/jhi.9.3.149-157.

Langeveld SA, Dore JM, Memelink J. 1991. Identification of potyviruses using the polymerase chain reaction with degenerate primers. J General Virology. 72:1531–1541. DOI: https://doi.org/10.1099/0022-1317-72-7-1531.

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum. 15(3):473–497. DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x.

Nasution SS, Dinarti D, Hidayat SH. 2017. Pengaruh elektroterapi dan termoterapi secara in vitro terhadap eliminasi Onion yellow dwarf virus. J Fitopatol Indones. 13(6):199–206. DOI: https://doi.org/10.14692/jfi.13.6.199.

Noveriza R, Suastika G, Hidayat SH, Kartosuwondo U. 2012. Eliminasi Potyvirus penyebab penyakit mosaik pada tanaman nilam dengan kultur meristem apikal dan perlakuan air panas pada setek batang. J. Littri. 18(3):107–114. DOI: https://doi.org/10.21082/jlittri.v18n3.2012.107-114.

Shahraeen N, Lesemann DE, Gholbi T. 2008. Survey for viruses infecting onion, garlic and leek crops in Iran. Eppo Bulletin. 38(1):131–135. DOI: https://doi.org/10.1111/j.1365-2338.2008.01198.x.

Sulistio M, Sulistyaningsih E, Subandiyah S. 2015. Elimination of shallot bulb viruses through heat treatment. Indo J Biotechnol. 20(2):133–140. DOI: https://doi.org/10.22146/ijbiotech.24196.

Supenti, Marlina N. 2011. Teknik pembebasan virus pada tanaman krisan. Bul Tek Pert. 16(2):64–67.

Sutarya R, Van Vreden, Korlina E, Gunaeni N, Duriat AS. 1993. Survei virus bawang merah pada beberapa lokasi di Kabupaten Brebes, Jawa Tengah. Bul Penel Hort. 26(1):97–106.

Wulandari AW, Hidayat SH, Sobir. 2015. Deteksi virus pada bawang merah (Allium cepa var. ascalonicum) dengan metode Dot Immuno Binding Assay. J Hort. 25(4):350–356. DOI: https://doi.org/10.21082/jhort.v25n4.2015.p350-356

Published
2021-05-06
How to Cite
Wulandari, A. W. W., Sobir, S., & Hidayat, S. H. (2021). Pengaruh Kombinasi Perlakuan Air Panas dan Kultur Jaringan terhadap Infeksi Virus pada Bawang Merah. Jurnal Fitopatologi Indonesia, 17(2), 49-57. https://doi.org/10.14692/jfi.17.2.49-57
Section
Articles