Keefektifan Bakteri Asal Lahan Gambut sebagai Agens Pengendalian Penyakit Kresek dan Pupuk Hayati pada Tanaman Padi

  • Giyanto Giyanto Department of Plant Protection, IPB University
  • Ali Nurmansyah Department of Plant Protection, IPB University
Keywords: antagonist agents, antibiosis, hypersensitive response (HR), leaf blight, Xanthomonas oryzae pv. oryzae

Abstract

Effectiveness of Bacterial from Peatlands as Biological Control Agents of Bacterial Leaf Blight Disease and Bio-Fertilizer in Rice

The use of microbes for disease control and biological fertilizers has been developed as an alternative to the development of environmentally friendly agriculture. Exploration of microbes from various types of ecological habitats is continuously being carried out to obtain the potential microbes.  Peatlands are thought to contain many types of microbes, especially bacteria that may play a role as antagonistic agents as well as biological fertilizers that are beneficial to plants. This study aimed to obtain bacterial strains that have the potential to control bacterial leaf blight (BLB) diseases on rice caused by Xanthomonas oryzae pv. oryzae as well as biological fertilizer. Research methodologies included sampling, isolating bacteria from peatlands and testing their potential as biological agents against X. oryzae pv. oryzae, biosafety tests, characterization of potential strains, test of the effectiveness of bacteria as antagonistic agents as well as biological fertilizers in planta. As many as 134 bacteria isolates were isolated from peat forest soil and former paddy fields and 40 of them showed different morphological characteristics. A total of 14 bacterial strains from peat soil were able to suppress the development of X. oryzae pv. oryzae. Characterization of 14 bacterial strains against the hypersensitive response (HR) test, blood agar hemolysis, nitrogen fixing ability, the resulting siderophore, phosphate solvent and cellulose degradation resulted in four potential bacterial strains. They have no potential as a pathogen for plants or mammals and have the character of a biological fertilizer. The in planta test of these four bacterial strains showed that these bacteria were able to reduce the severity of BLB disease in rice and increase rice growth in the vegetative phase.

Downloads

Download data is not yet available.

Author Biography

Ali Nurmansyah, Department of Plant Protection, IPB University

-

References

Goldstein AH. 1995. Recent progress in understanding the molecular genetics and biochemistry of calcium phosphate solubilisation by Gram-negative bacteria. Biol Agric Hort. 12:185–193. DOI: https://doi.org/10.1080/01448765.1995.9754736.

Gupta P, Samant K, Sahu A. 2012. Isolation of cellulose-degrading bacteria and determination of their cellulolytic potential. Inter J Microbiol. 2012:1–5. DOI: https://doi.org/10.1155/2012/578925.

Hoa PTP, Quang ND, Sakiyama Y, Hop DV, Hang DT, Ha TH, Van NT, Quy NTK, Da NTA. 2012. Sreening for actinomyces isolated from soil with the ability to inhibit Xanthomonas oryzae pv. oryzae causing rice blight disease in Vietnam. Afr J Biotechnol. 11(80):14586–14594.

IRRI (International Rice Research Institute) 2014. Standard Evaluation System for Rice. 5th Ed. Los Banos (PH): INGER Genetic Resources Center. Hlm 55.

Karpagam T, Nagalakshmi PK. 2014. Isolation and characterization of phosphate solubilizing microbes from agricultural soil. Inter J Current Microbiol Appl Sci. 3(3):601–614.

Kifle MH, Laing MD. 2016. Isolation and screening of bacteria for their diazotrophic potential and their influence on growth promotion of maize seedlings in greenhouses. Frontiers Plant Sci. 6(1):1–8. DOI: http:10.3389/fpls.2015.01225.

Kim KY, Jordan D, Mc Donald GA. 1997. Solubilization of hydroxyl apatite by Enterobacter agglomerans and cloned Escherichia coli in culture medium. Biol Fertil Soils. 24:347–352. DOI: https://doi.org/10.1007/s003740050256.

Liu B, Talukder MJH, Terhonen E, Lampella M, Vasander H, Sun H, Asiegbu F. 2019. The microbial diversity and structure in peatland forest in Indonesia. Soil Use Manage. 36:123–138. DOI: https://doi.org/10.1111/sum.1254.

Mace GM, Norris K, Fitter AH. 2012. Biodiversity and ecosystem services: a multilayered relationship. Trends Ecol Evol. 27:19–26. DOI: https://doi.org/10.1016/j.tree.2011.08.006.

Milagres AMF, Machuca A, Napoleao D. 1999. Detection of siderophore production from several fungi and bacteria by a modification of chrome azurol S (CAS) agar plate assay. J Microbiol Methods. 37(1999):1–6. DOI: https://doi.org/10.1016/S0167-7012(99)00028-7.

Pankratov TA, Ivanova AO, Dedysh SN, Liesack W. 2011. Bacterial populations and environmental factors controlling cellulose degradation in an acidic sphagnum peat. Environ Microbiol. 13:1800–1814. DOI: https://doi.org/10.1111/j.1462-2920.2011.02491.x.

Pratiwi E, Satwika TD, Agus F. 2018. Keanekaragaman mikroba tanah gambut di bawah hutan dan di bawah perkebunan sawit di Provinsi Jambi. J Tanah Iklim 42:69–78. DOI: https://doi.org/10.21082/jti.v42n1.2018.69-78.

Ritung S, Wahyunto, Nugroho K, Sukarman, Hikmatullah, Suparto, Tafakresnanto C. 2011. Peta Lahan Gambut Indonesia Skala 1:250.000. Bogor (ID): Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian.

Roselló-Mora R, Amann R. 2001. The species concept for prokaryotes. FEMS Microbiol Rev. 25:39–67. DOI: https://doi.org/10.1111/j.1574-6976.2001.tb00571.x.

Schaad W, Jones JB, Chun W. 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria, Third Edition. St Paul (US): APS Press.

Sorensen KW. 1993. Indonesian peat swamp forests and their role as a carbon sink. Chemosphere. 27:1065–1082. DOI: http://dx.doi.org/10.1016/0045-6535(93)90068-G.

Suparyono S, Suprihanto. 2004. Pathotype profile of Xanthomonas oryzae pv. oryzae isolates from the rice ecosystem in Java. Indones J Agric Sci. 5(2):63–69. DOI: https://doi.org/10.21082/ijas.v5n2.2004.63-69.

UNDP. 2006. Malaysia’s Peat Swamp Forest Conservation and Sustainable Use. Kualalumpur (MY): UNDP.

Published
2021-05-06
How to Cite
Giyanto, G., & Nurmansyah, A. (2021). Keefektifan Bakteri Asal Lahan Gambut sebagai Agens Pengendalian Penyakit Kresek dan Pupuk Hayati pada Tanaman Padi. Jurnal Fitopatologi Indonesia, 17(2), 67-75. https://doi.org/10.14692/jfi.17.2.67-75
Section
Articles