Characterization of Phenol Degrading Bacteria from Buntal Lake of Central Kalimantan

  • Nursaadah .
  • Dwi Andreas Santosa
  • Maggy T Suhartono

Abstrak

Three bacterial isolates (ICBB 1168, ICBB 1169, ICBB 1170), being capable to utilizing phenol as sole carbon and energy source, were isolated from Buntal Lake of Central Kalimantan. Growth of all isolates were optimum at 37OC. Optimum pH for growth and phenol degradation of ICBB 1168, lCBB 1169, and ICBB 1170 were 7-8, 6, and 6-7, respectively. Among the isolates, ICBB 1170 showed best phenol degrading activity. ICBB 1170 able to degrade 16 mM phenol to 0.71 mM in 4
days. Phenol degradation ability of ICBB 1170 could be increased by adding 0.01-0.1% yeast extract. Addition of 0.05-0.1% glucose in medium inhibited phenol degradation by ICBB 1170. Cell-free extracts of ICBB 1170 had specific activity 2.92 Ulmg. Degradation of benzoate by ICBB 1170 was
studied. However, the ability to degrade phenol were higher than that of benzoate.

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Nursaadah .
Jurnal Tanah dan Lingkungan, dengan ISSN 1410-7333 diterbitkan dua kali setahun yaitu pada bulan April dan Oktober oleh Departemen Ilmu Tanah dan Sumberdaya Lahan (nama baru dari Departemen Tanah), Fakultas Pertanian, Institut Pertanian Bogor. Jurnal Tanah dan Lingkungan menyajikan artikel mengenai hasil penelitian dan ulasan tentang perkembangan mutakhir dalam bidang ilmu tanah, air dan ilmu lingkungan sebagai bahan kajian utama. Setiap naskah yang dikirim ke Jurnal Tanah dan Lingkungan, akan ditelaah oleh penelaah (reviewer) yang sesuai dengan bidangnya. Nama penelaah dicantumkan pada terbitan No. 2 dari setiap volume
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2001-10-01