Growth and Extracelluler Polysaccaride Production of Porphyridium cruentum In Various Photoperiod

  • Himawan Prasetyo Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, IPB Kampus IPB Darmaga, Jalan Agatis, Bogor 16680 Jawa Barat, Indonesia Telepon (0251)8622909-8622906, Faks. (0251)8622907
  • Iriani Setyaningsih Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, IPB Kampus IPB Darmaga, Jalan Agatis, Bogor 16680 Jawa Barat, Indonesia Telepon (0251)8622909-8622906, Faks. (0251)8622907
  • Dewi Ratih Agungpriyono Departemen Klinik Reproduksi dan Patologi, Fakultas Kedokteran Hewan, IPB Kampus IPB Darmaga, Jalan Agatis, Bogor 16680 Jawa Barat, Indonesia Telepon (0251)8629469-8629470, Faks. (0251)8629459
Keywords: Biomass, growth, photoperiod, polysaccharides, Porphyridium cruentum

Abstract

Porphyridium cruentum is a kind of red microalgae (Rhodophyta) that can grow and produce
polysaccharides. Polysaccharides are produced on the cell, then released and accumulated by
extracellular into medias. This study aimed to determine the optimum growth and production
of extracellular polysaccharides (EPs) from P. cruentum in various photoperiods. The cultivation
was done with the photoperiods at 24:0, 18:6, 12:12 and 06:18 h (light:dark) during 40 days using
modified medium F/2. The growth is determined by measuring optical density (OD) and dried
biomasses, the productions of EPs are determined by dried weight polysaccharides as a result
of precipitation. The results showed that OD at photoperiod 24:0 has the highest value 0.876 ±
0.0645 unit of absorbance, biomass concentrations are highest in the photoperiod 24:0 and 12:12
respectively 716.12 ± 123 mg-l and 696.5 ± 74.5 mg-l, the highest production of EPs on the
photoperiod 12:12 is 1.310 ± 130.26 mg-l. 12:12 hours (light: dark) photoperiod is optimally used
in P. cruentum cultivations with high biomass and extracellular polysaccharide results.

Published
2015-12-19