Oil Palm Phytochrome-Interacting Factor4 (PIF4) Gene is Conserved and Highly Expressed During Somatic Embryogenesis

  • Mantira Suksirt Department of Genetics, Faculty of Science, Kasetsart University, Bangkok
  • Kamolwan Khianchaikhan Department of Genetics, Faculty of Science, Kasetsart University, Bangkok
  • Mya Thuzar Plant Biotechnology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC-NSTDA), Pathumthani
  • Supachai Vuttipongchaikij Department of Genetics, Faculty of Science, Kasetsart University, Bangkok Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart (CASTNAR, NRU-KU), Kasetsart University, Bangkok
  • Chatchawan Jantasuriyarat Department of Genetics, Faculty of Science, Kasetsart University, Bangkok Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart (CASTNAR, NRU-KU), Kasetsart University, Bangkok
Keywords: oil palm, PHYTOCHROME-INTERACTING FACTOR4, somatic embryogenesis, tissue culture

Abstract

Oil palm is used in food, fuel and cosmetic industries. Tissue culture is the best way to propagate oil palm; unfortunately the somatic embryogenesis during tissue culture takes long time. The molecular mechanism of somatic embryogenesis in oil palm remains unknown. Recent research reported that auxin plays an important role in early and post-embryogenic plant. PHYTOCHROME-INTERACTING FACTOR4 (PIF4) regulates levels of auxin and the expression of key auxin biosynthesis genes. Our research aims to characterize oil palm PIF4 gene. Thus, we cloned EgPIF4, analyzed the domain using bioinformatic and examined the expression of EgPIF4 during somatic embryogenesis at different tissue including callus and somatic embryo stages; globular, torpedo, cotyledon, and plantlet stage using real-time PCR method. The result showed that EgPIF4 gene comprised 1,737 bp with 9 exons, which encode 578 amino acid residuals. It contains a conserved domain called basic helix-loop-helix domain. EgPIF4 has high level of expression at somatic embryogenetic stage specifically globular and torpedo stage suggested that EgPIF4 plays an important role during somatic embryogenesis. The future characterization of EgPIF4 function in oil palm will help to understand somatic embryogenesis process and facilitate the improvement of the oil palm tissue culture.

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Published
2019-12-28