Phytochemical Analysis, α-glucosidase Inhibition Activity in-vitro and Enzyme Kinetics of Ethyl Acetate and Hexane Extracts of Graptophylum pictum (L.) Griff

Waras Nurcholis, I Made Artika, Djarot Sasongko Hami Seno, Dimas Andrianto, Apipah Aprianti, Fina Febrianti, Inawati Inawati, Antonius Padua Ratu, Arya Arendra

Abstract


The species Graptophylum pictum (L.) Griff, also known as “daun ungu” in Indonesia, is a traditional herbaceous plant believed to have antidiabetic potential. The number of people in the world with diabetes has increased dramatically over the recent years. The treatment of type II diabetes is complicated by several factors inherent to the disease. Elevated postprandial hyperglycemia is one of the risk factors and the intestinal digestive enzyme α-glucosidase plays a vital role in carbohydrate metabolism. One of the antidiabetic therapeutic approaches which reduces the postprandial glucose level in blood is by the inhibition of α-glucosidase. In this study, phytochemical analysis, α- glucosidase inhibitory activity and enzyme kinetics of ethyl acetate- and hexane extracts of G. pictum were evaluated with the aim to analyze its antidiabetic potential. Phytochemical analysis revealed the presence of tannins, steroids, and alkaloids. Steroids were present in ethyl acetate extract but absent in hexane extract, while alkaloids were present in hexane extract but absent in ethyl acetate extract. The ethyl acetate and hexane extracts had 30.68 and 49.82 % inhibitory effect on α-glucosidase activity respectively. The kinetics of glucosidase enzyme of ethyl acetate and hexane extracts were determined by Lineweaver Burk plots. These exhibited uncompetitive and noncompetitive inhibition to alpha-glucosidase activity respectively. From the enzyme assay, we infer that ethyl acetate and hexane extracts of G. pictum contain potential α-glucosidase inhibitors that have the potential to be exploited for use in the treatment of diabetes


Keywords


Graptophyllum pictum (L.) Griff; α-glucosidase inhibitor activity; kinetics; diabetes

Full Text:

PDF

References


Ani V, Akhilender NK. 2008. Antihyperglycemic activity of polyphenolic components of black/bitter cumin Centratherum anthelminticum (L.) Kuntze seeds. Eur. Food. Res. Technol. 226: 897-903.

Bhat M, Zinjarde SS, Bhargava SY, Kumar AR, Joshi BN. 2011. Antidiabetic Indian Plants: A Good Source of Potent Amylase Inhibitors. Evidence-Based Compl. Alt. Medicine.:1-6.

Danaei G, Finucane MM, Lu Y, Singh GM, Cowan MJ, Paciorek CJ. 2011. National, regional, and global trends in fasting plasma glucose and diabetes prevalence since 1980: systematic analysis of health examination surveys and epidemiological studies with 370 country-years and 2.7 million participants. Lancet. 378:31–40.

Gin H, Rigalleau V. 2000. Post-prandial hyperglycemia and Diabetes. Diabet. Metabol. 26:265-72.

Gray DM. 1995. Carbohydrate digestion and absorption—role of small intestine. New. Eng. J. Med. 29: 1225–1230.

Gurudeeban S, Satyavani K, Ramanathan T. 2012. Alpha glucosidase inhibitory effect and enzyme kinetics of coastal medicinal plants. Bangladesh. J. Pharmacol. 7: 186-191.

Harborne JB. 1998. Phytochemical Methods. London: Chapman and Hall.

Horii S, Fukasse K, Matsuo T, Kameda K, Asano N, Masui Y. 1987. Synthesis and α-D-glucosidase inhibitory activity of N-substituted valiolamine derivatives as potent oral antidiabetic agents. J. Med. Chem. 29: 1038–1046.

Hua-Qiang D, Mei L, Feng Z, Fu-Lai L, Jian-Bo H. 2012. Inhibitory potential of trilobatin from Lithocarpus polystachyus Rehd against α-glucosidase and α -amylase linked to type 2 diabetes. Food. Chem. 130: 261–266.

Kaskoos RA. 2013. In-vitro α-glucosidase inhibition and antioxidant activity of methanolic extract of Centaurea calcitrapa from Iraq. Ame. J. Essent. Oils Nat. Prod. 1: 122-125.

Kwon YI, Vattem DA, Shetty K. 2007. Evaluation of clonal herbs of Lamiaceae species for management of diabetes and hypertension. Asia. Pac. J. Clin. Nutr. 15:107-118.

Lineweaver H, Burk D. 1934. The determination of enzyme dissociation constants. J. Am. Chem. Soc. 56: 658-666.

Mayur B, Sandesh S, Shruti S, Sung-Yum S. 2010. Antioxidant and α- glucosidase inhibitory properties of Carpesium abrotanoides L. J. Med. Plant. Res. 4: 1547-1553.

Nagmoti DM, Juvekar AR. 2013. In vitro inhibitory effects of Pithecellobium dulce (Roxb.) Benth. seeds on intestinal α-glucosidase and pancreatic α-amylase. J. Biochem. Tech. 4: 616-621.

Nurcholis W, Andrianto D, Falah S. Katayama T. 2011. α-Glucosidase inhibitor and cytotoxic activities and phytochemical screening of Graptophylum pictum (L.) Griff. The 3rd International Symposium of Indonesian Wood Research Society. Jogjakarta, November 3-4, 2011.

Olangbede-Dada SO, Ogbonnia SO, Coker HAB, Ukpo GE. 2011. Blood glucose lowering effect of aqueous extract of Graptophyllum pictum (Linn) Griff. on alloxan-induced diabetic rats and its acute toxicity in mice. Afr. J. Biotechnol. 10: 1039-1043.

Ozaki Y, Sekita S, Soedigdo S, Harada M. 1989. Anti-inflammatory effect of Graptophyllum pictum (L.) Griff. Chem. Pharm. Bull. 37: 2799-2802.

Pogano G, Marena S, Corgiant-Mansin L, Cravero F, Giorda C, Bozza M. 1995. Comparison of miglitol and glibenclamide in diet-treated type 2 diabetic patients. Diabetes. Metab. 21: 162-167.

PT Eisai Indonesia editor. 1995. Medicinal herbs index in Indonesia. PT Eisai Indonesia editor, Jakarta-Indonesia.

Pulok KM, Kuntal M, Kakali M, Peter JH. 2006. Leads from Indian medicinal plants with hypoglycemic potentials. J. Ethnopharmacol. 106: 1–28.

Rahimi R, Nikfar S, Larijani B, Abdollahi M. 2005. A review on the antioxidants in the management of diabetes and its complications. Biomed. Pharmacother. 59: 365-373.

Schmidt D, Former H, Junge B, Muller M, Wingender W, Trusheit E. 1977. A-glucosidase inhibitor: New complex oligosaccharides of microbial origin. Naturwissenschaften. 64: 535–536.

Shibano M, Kitagawa S, Nakamura S, Akazawa N, Kusano G. 1997. Studies on the constituents of Broussonetia species. II. Six new pyrrolidine alkaloids, broussonetine A, B, E, F and broussonetinine A and B, as inhibitors of glycosidases from Broussonetia kazinoki Sieb. Chem. Pharm. Bull. 45:700-705.

Shim YJ, Doo HK, Ahn SY, Kim YS, Seong JK, Park IS. 2003. Inhibitory effect of aqueous extract from the gal of Rhuz chinesis on alpha-glucosidase activities and postprandial blood glucose. J. Ethnopharmacol. 85: 283– 287.

Suzuki Y, Sano M, Hayashida K, Ohsawa I, Ohta S, Fukuda K. 2009. Are the effects of alphaglucosidase inhibitors on cardiovascular events related to elevated levels of hydrogen gas in the gastrointestinal tract?. FEBS. Letters. 583:2157-2159.

WHO [World Health Organization]. 2009. Global health risks. Mortality and burden of disease attributable to selected major risks. Geneva, World Health Organization.

WHO [World Health Organization]. 2011. Global status report on noncommunicable diseases 2010. Geneva, World Health Organization.




DOI: https://doi.org/10.29244/cb.1.2.58-65

Refbacks

  • There are currently no refbacks.


Editorial Office :

Current Biochemistry
Department of Biochemistry, Bogor Agricultural University
Jln Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia
Phone/Fax.: +62-251-8423267
E-mail: current.biochemistry@gmail.com